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KMID : 0880520110470020072
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Chonnam Medical Journal
2011 Volume.47 No. 2 p.72 ~ p.79
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Interplay of Hydrogen Sulfide and Nitric Oxide on the Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine
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Yoon Pyung-Jin
Shanker Prasad Parajuli
Zuo Dong-Chuan
Pawan Kumar Shahi
Oh Hyung-Jung
Shin Hae-Rang
Lee Mi-Jung
Yeum Cheol-Ho
Choi Seok
Jun Jae-Yeoul
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Abstract
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We studied whether nitric oxide (NO) and hydrogen sulfide (H2S) have an interaction on the pacemaker activities of interstitial cells of Cajal (ICC) from the mouse small intestine. The actions of NO and H2S on pacemaker activities were investigated by using the whole-cell patch-clamp technique and intracellular Ca2+ analysis at 30℃ in cultured mouse ICC. Exogenously applied (±)-S-nitroso-N-acetylpenicillamine (SNAP), an NO donor, or sodium hydrogen sulfide (NaHS), a donor of H2S, showed no influence on pacemaker activity (potentials and currents) in ICC at low concentrations (10 μM SNAP and 100 μM NaHS), but SNAP or NaHS completely inhibited pacemaker amplitude and pacemaker frequency with increases in the resting currents in the outward direction at high concentrations (SNAP 100 μM and NaHS 1 mM). Co-treatment with 10 μM SNAP plus 100 μM NaHS also inhibited pacemaker amplitude and pacemaker frequency with increases in the resting currents in the outward direction. ODQ, a guanylate cyclase inhibitor, or glibenclamide, an ATP-sensitive K+ channel inhibitor, blocked the SNAP+NaHS-induced inhibition of pacemaker currents in ICC. Also, we found that SNAP+NaHS inhibited the spontaneous intracellular Ca2+ ([Ca2+]i) oscillations in cultured ICC. In conclusion, this study describes the enhanced inhibitory effects of NO plus H2S on ICC in the mouse small intestine. NO+H2S inhibited the pacemaker activity of ICC by modulating intracellular Ca2+. These results may be evidence of a physiological interaction of NO and H2S in ICC for modulating gastrointestinal motility.
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KEYWORD
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Interstitial cells of Cajal (ICC), Intestinal motility, Pacemaker currents, Nitric Oxide, Hydrogen sulfide
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